李元辉;

• 1:国药集团药业股份有限公司

摘要(Abstract)：

目的测定橙皮素的大鼠血浆蛋白结合率。方法采用平衡透析法结合高效液相色谱法(HPLC)对橙皮素与大鼠血浆的血浆蛋白结合率进行测定。结果在0.5、2.5和10μg/m L浓度水平橙皮素与大鼠血浆的血浆蛋白结合率分别为(93.5±4.2)%,(94.3±5.6)%和(93.9±3.8)%,三个浓度水平之间的血浆蛋白结合率差异无统计学意义(P≥0.05)。结论橙皮素与大鼠血浆蛋白高度结合。

关键词(KeyWords)： 橙皮素;血浆蛋白结合率;大鼠;平衡透析法;高效液相色谱法

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李元辉;

Email:

DOI: 10.14053/j.cnki.ppcr.201509020

参考文献(References)：

• [1]刘学仁,张莹,林志群.橙皮苷和橙皮素生物活性的研究进展[J].中国新药杂志,2011,20(4):329-333.
• [2]Griffiths LA,Smith GE.Metabolism of apigenin and related compounds in the rat metabolite formation in vivo and by the intestinal microflora in vitro[J].Biochem,1972,128(4):901-911.
• [3]王喜军,陈曦,曹洪欣,等.口服枳术丸后人体内橙皮苷、柚皮苷的药代动力学研究[J].中国实验方剂学杂志,2007,13(8):18-20.
• [4]Choiem EM,Kim YH.Hesperetin attenuates the highly reducing sugar-rtiggeed inhibition of osteoblast differentiation[J].Cell Biol Toxicol,2008,24(3):225-231.
• [5]Cho J.Antioxidant and neuroprotective effects of hesperidin and itsaglyconehesperetin[J].Arch Pharm Res,2006,29(8):699-706.
• [6]Orallof F,Alvarez E,Basaram H,et al.Comparative study of the vasorelaxant activity,superoxide-scavenging ability and cyclic nucleotide phosphodiesterase-inhibitory effects of hesperetin and hesperidin[J].Naunyn-Schmiedebergs Arch Pharmacol,2004,370(6):452-463.
• [7]Otagiri M.Study on binding of drug to serum protein[J].Yakugakuzasshi:Journal of the Pharmaceutical Society of Japan,2009,129(4):413-425.
• [8]Howard ML,Hill JJ,Galluppi GR,et al.Plasma protein binding in drug discovery and development[J].Combinatorial Chemistry&High Throughput Screening,2010,13(2):170-187.
• [9]万丽丽,张剑萍,孙习鹏,等.HPLC法测定大鼠血浆中橙皮素和柚皮素的浓度[J].中国药师,2009,12(11):1521-1523.
• [10]钟大放.以加权最小二乘法建立生物分析标准曲线的若干问题[J].药物分析杂志,1996,16:343-346.