白雪;彭扬;李慧;张月;何平;

• 1:中国医科大学附属盛京医院

摘要(Abstract)：

目的探讨山楂酸与顺铂合用对肺癌细胞A549增殖及凋亡产生的影响。方法将人的肺癌细胞系A549经过药物干预(山楂酸、顺铂),并将干预后的肺癌细胞通过MTT实验,分析细胞增殖能力变化,通过流式细胞技术,分析细胞的凋亡水平变化。通过Western blot分析细胞中XIAP、Survivin的表达水平变化。结果山楂酸和顺铂合用后,A549细胞的增殖率显著降低,凋亡水平显著提高;荧光电子显微镜下观察到凋亡细胞明显增加,核碎裂明显,细胞大片脱落;Western blot结果显示,山楂酸和顺铂合用,显著下调XIAP、Survivin的蛋白表达量。结论山楂酸和顺铂合用抑制肺癌细胞的增殖,促进肺癌细胞的凋亡,并下调细胞中凋亡抑制相关指标XIAP、Survivin的表达水平。

关键词(KeyWords)： 肺癌;山楂酸;顺铂;增殖;凋亡

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 白雪;彭扬;李慧;张月;何平;

Email:

DOI: 10.14053/j.cnki.ppcr.201704003

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